Developmental Studies Hybridoma Bank

As a National Resource created by the NIH, the Developmental Studies Hybridoma Bank (DSHB) remains true to its basic mission, to distribute mAbs and hybridoma cell lines at cost. The price is around $40 per ml of supernatant. A new website format includes thumbnail images in the general catalog for each DSHB mAb. The thumbnail images in most cases are fluorescently stained samples, but in some cases include another application such as a western. Each image includes at the bottom the name and institution of the scientist who provided it (e.g., D. Soll, UIOWA).  There is also an NCI initiative regarding the RAS signaling pathways. These antibodies were generated against 27 phosphopeptides and 69 unmodified peptides, representing 20 RAS network proteins, and are ready for distribution through the DSHB. The list of these 20 targets (red), as well as DSHB mAbs against other RAS pathway targets (black), is presented below.                     
AKT1AKT2AKT3ARAFBRAF
CCND1CDH1CDH2DUSP7E2F4
E2F6E2F8EGFRERBB2ERBB3
ETS1ETS2FOSGSK3BJUN
MAP2K1MAPK1MAPK3MTORNFE2L2
NFKB1PAK1PEBP1PIK3R1PIK3R2
PLXNB1PTENRAC1RB1RPTOR
SHC4TP53YAP1